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Biochemical assay demonstrating interactions between 6×His–Ino4p and radioactively labeled bHLH proteins. Each of the labeled bHLH proteins was incubated with extracts from E.coli programmed with either pET-15b-INO4 (+) or pET-15b (–). The input lanes contain 20% of the labeled bHLH proteins used in the reactions. The predicted molecular weights for each of the bHLH proteins are shown.
