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CHO1–lacZ expression in response to hyperosmotic stress and growth phase. (A) Cultures of strain BRS1189 were grown in synthetic medium: lacking inositol and choline (open squares); containing 75 µM inositol and 1 mM choline (solid squares); containing 0.7 M NaCl (open circles); containing inositol, choline and NaCl (solid circles). Aliquots were taken at various times throughout the growth phase. (B) Klett readings were taken for each aliquot.
