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. 2024 Aug 6;90(11):844–857. doi: 10.1055/a-2353-1469

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The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ )

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 5 — ROS accumulation and malondialdehyde levels. In panel a , the accumulation of ROS measured with the cytofluorometer is shown. Each box refers to a treatment as indicated: the x-axis represents the fluorescence of the fluorochrome Fitc connected to our fluorescent probe, while the y-axis is relative to the number of cells that we decided to acquire (10 000). At the top of each box, there is a marker (M3), which is arbitrarily drawn in the control and kept the same for all other samples. The part of the peak included in M3 is indicated by a numerical percentage. In panel b , the respective quantification, obtained from comparing the percentages, is represented. The control percentage is arbitrarily made equal to 1 and the other values are related to it. Panel c represents the levels of malondialdehyde generated by the different treatments. Three independent experiments were carried out, and the values are expressed as the mean ± standard deviation (sd). * denotes p < 0.05 vs. CTRL; ** denotes p < 0.01 vs. CTRL; § denotes p < 0.05 vs. lead. Analysis of variance (ANOVA) was followed by a Tukey–Kramer comparison test.