Figure 2.

SDS–PAGE of fractions recovered during the purification of rI-CmoeI. These fractions consist of the lysate of cells induced with IPTG (Lysate), the soluble fraction of the lysate (Soluble), the fraction eluted from the Ni–NTA resin (Ni-NTA) and the pool of fractions eluted from the S-ceramic Hyper D column (Hyper D). Proteins were electrophoresed in a 12% polyacrylamide gel and stained with Coomassie brilliant blue. Proteins corresponding to 100 µl of bacterial culture were loaded in the first three lanes; the last lane contains 2.5 µg of purified protein. The arrowhead denotes the position of the rI-CmoeI protein.