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Effect of mutation H154A on DNA binding. Protein–DNA complexes were formed in reaction mixtures containing 10, 30, 100 and 300 nM wild-type I-CmoeI (W) or mutant H154A (M) and 10 nM 33P-labeled DNA substrate. As a control, a mixture containing only the DNA substrate was also electrophoresed in the 5% polyacrylamide gel (lane S).
