REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti CYP51A1 peptide QRLKDSWAERLDFNPDRY	Custom synthesized (Keber et al.8)	anti CYP51A1
Recombinant Anti-Seladin 1 antibody	Abcam	ab181062; RRID:AB_2832945
Anti-SC5DL antibody	Abcam	ab221764
Anti-SREBP2	Thermo Fisher Scientific	PA1-338; RRID:AB_2194237
Anti-Wnt5a/b	Cell Signalling Technology	C27E8; RRID:AB_2215595
Anti-Dvl3	Cell Signalling Technology	3218; RRID:AB_10694060
Anti-Dvl2 (30D2)	Cell Signalling Technology	3224; RRID:AB_2093336
Anti-LRP6 (C47E12)	Cell Signalling Technology	3395; RRID:AB_1950408
Anti- Phospho-LRP6 (Ser1490)	Cell Signalling Technology	2568; RRID:AB_2139327
Chemicals, peptides, and recombinant proteins
lanosterol	Avanti Polar Lipids	Cat#700063
24,25-dihydrolanosterol	Avanti Polar Lipids	Cat#700067
T-MAS	Avanti Polar Lipids	Cat#700073
FF-MAS	Avanti Polar Lipids	Cat#700177
dihydro-T-MAS	Avanti Polar Lipids	Cat#700173
dihydro-FF-MAS	Avanti Polar Lipids	Cat#700173
zymosterol	Avanti Polar Lipids	Cat#700068
zymostenol	Avanti Polar Lipids	Cat#700118
24-dehydrolathosterol	Avanti Polar Lipids	Cat#700114
lathosterol	Avanti Polar Lipids	Cat#700069
7-dehydrodesmosterol	Avanti Polar Lipids	Cat#700138
desmosterol	Avanti Polar Lipids	Cat#700060
cholesterol	Merck	Cat#C3045
lathosterol-D7	Avanti Polar Lipids	Cat#700056
Lanosterol-D6	Avanti Polar Lipids	Cat#700090
24(S/R) 25-epoxylanosterol	Avanti Polar Lipids	Cat#792747
Lipid depleted serum (LDS)	Biosera inc	Cat#FB-1001L/100
TRI Reagent® RNA Isolation Reagent	Sigma-Aldrich	Cat# T9424
Critical commercial assays
Agilent RNA 6000 Nano Kit	Agilent Technologies	Cat# 5067-1511
Human Clariom™ S Assay	Thermo Fisher Scientific	Cat# 902927
QuantiNova Reverse Transcription Kit	Qiagen	Cat# 205411
SYBR Green I Master	Roche	Cat# 04887352001
No-Stain Protein Labeling Reagent	Invitrogen	Cat#A44717
Deposited data
Raw and analyzed data	This paper	GEO GSE221582
Experimental models: Cell lines
HepG2 cell line	Synthego/This paper	Native
HepG2 CYP51A1 KO	Synthego/This paper	CYP51 KO
HepG2 DHCR24 KO	Synthego/This paper	DHCR24 KO
HepG2 SC5D KO	Synthego/This paper	SC5D KO
Software and algorithms
GraphPad Prism 10.2.2	https://www.graphpad.com	GraphPad Software
Venny 2.0	https://bioinfogp.cnb.csic.es/tools/venny/index.htm	Venny 2.0
Biorender	https://www.biorender.com/	Biorender
ToppGene Suite	https://toppgene.cchmc.org/	ToppGene Suite