Figure 6.

Mitochondrial telomeric minicircles of P.philodendra and C.salmanticensis isolated by alkaline lysis of purified mitochondria. Mitochondrial telomeric minicircles of P.philodendra (A–D) and C.salmanticensis (E–G) were isolated from purified mitochondria (Materials and Methods). Due to the very small size of some circles, the samples were directly adsorbed to thin carbon foils in the absence of denatured protein, and rotary shadowcast with tungsten (Materials and Methods). Shown in reverse contrast. Bar represents 0.1 µm. The P.philodendra examples have contour lengths 300 bp (A), 600 bp (B), 0.9 kb (C) and 1200 bp (D), respectively. The C.salmanticensis examples have contour lengths of 200 bp (E), 400 bp (F) and 600 bp (G), respectively. (H) Histogram of circle lengths measured from micrographs as in (A)–(D). (I) A histogram of circle lengths measured from micrographs as in (E)–(G).