Table 1.

Genes used in this study with PCR primers and optimal annealing temperature.

Locus	PCR Primers	PCR: Thermal Cycles: (Annealing Temp. in Bold)	Reference
ITS	ITS1/ITS4	(95 °C: 30 s, 51 °C: 30 s, 72 °C: 1 min) × 35 cycles	White et al. (1990)
act	ACT-512F/ACT-783R	(95 °C: 45 s, 55 °C: 45 s, 72 °C: 1 min) × 35 cycles	Carbone and Kohn (1999)
chs1	CHS-79F/CHS-345R	(95 °C: 30 s, 58 °C: 30 s, 72 °C: 1 min) × 35 cycles	Carbone and Kohn (1999)
gapdh	GDR1/GDF1	(95 °C: 30 s, 58 °C: 30 s, 72 °C: 1 min) × 35 cycles	Guerber et al. (2003)
his3	CYLH3F/CYLH3R	(95 °C: 30 s, 58 °C: 30 s, 72 °C: 1 min) × 35 cycles	Crous et al. (2004)
tub2	T1/Bt2b	(95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles	Glass and Donaldson (1995)