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. 2024 Aug 23;25(9):4062–4077. doi: 10.1038/s44319-024-00224-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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(A) Schematic illustration showing degradation of RAD21-mACl and SMC2-BTCh in the cohesin and condensin complexes, respectively. (B) Top, density plots of the cells treated with 1 µM 5-Ph-IAA, 0.5 µM AGB1 and both for 2 h. The X and the Y axes are the signal intensity of RAD21-mACl and SMC2-BTCh, respectively. Quadrant gates (Q1–Q4) were set manually to include 99.5% of the single cells in the Q4 area using a negative control (DMSO-treated WT cells). Bottom, the density plot data were quantified to make this graph. Data were presented as mean ± SD of three technical replicates (n = 3). The signal intensities in each condition were normalized with DMSO-treated control cells. (C) Representative DAPI-stained chromosomes from RAD21-mACl/SMC2-BTCh cells after treating them with the indicated ligand for 3 h. The scale bars indicate 10 µm. Source data are available online for this figure.