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. 2024 Aug 23;25(9):4062–4077. doi: 10.1038/s44319-024-00224-4

Figure 4. Enhancing reporter depletion by a double-degron system with AID2 and BromoTag.

Figure 4

(A) The reporter cells used in Figs. 2 and 3 were treated with 0.2 nM 5-Ph-IAA and/or 3.8 nM AGB1 for 4 h. GFP intensity was monitored taking the mock-treated cells as 100%. Data were presented as mean ± SD. Each dot represents a technical replicate (n = 3). (B) Depletion kinetics of the reporter depletion in cells treated with 1 µM 5-Ph-IAA, 0.5 µM AGB1, or both. The GFP intensity was monitored at 0, 15, 30, 60, 120, and 240 min taking the mock-treated cells as 100%. Data were presented as mean ± SD of three technical replicates (n = 3). The data were fitted with one-phase decay. Source data are available online for this figure.