Skip to main content
. Author manuscript; available in PMC: 2024 Sep 11.
Published in final edited form as: Transl Res. 2023 May 21;260:17–31. doi: 10.1016/j.trsl.2023.05.002

Fig 2.

Fig 2.

The impact of chronic hindlimb ischemia on intramuscular mitochondrial content varies based on animal obesity status. (A) Overall mitochondrial content assessed via citrate synthase (CS) activity. (B) Representative western blot of the individual mitochondrial electron transport chain complexes I − V in ischemic (Isch) and nonischemic (NI) quadricep muscle from mice fed a high-fat, high sucrose (HFS) or low-fat, low-sucrose (LFS) diet. Ponceau S staining represents total protein per lane used for normalization. Fold change of (C) Complex I (CI), (D) Complex II (CII), (E) Complex III (CIII), (F) Complex IV (CIV) and (G) Complex V (CV) protein expression relative to NI muscle from LFS-fed control mice. Two-way ANOVA with Tukey post-hoc tests used for analyzes. Data are mean ± SE; ns represents no significant difference, * P < 0.05, *** P < 0.001, **** P < 0.0001 (n = 10−12 mice per group).