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. 2024 Jul 25;27(9):110587. doi: 10.1016/j.isci.2024.110587

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© 2024 The Author(s)

This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

PMC Copyright notice

2D and 3D viability of renal cancers cells upon silencing of B7-H5

(A) Relative viability of 786-O and Caki-1 cells following transfection with siNS (negative control), siB7-H5 #1 or siB7-H5 #4. Data are presented relative to the negative control.

(B) 786-O and Caki-1 cell spheroid evolution the day of transfection (Day 1) and 3 days after transfection (Day 4).

(C) 3D viability of 786-O and Caki-1 cell spheroids after transfection with siNS, siB7-H5#1 or siB7-H5#4. Data are represented from one representative experiment out of three biological replicates, with 3–6 technical replicates, ±SD. Statistically significant results (p < 0.05) are marked with an asterisk.