REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-FLAG	Millipore	Cat#MAB3118; RRID: AB_11213007
Anti-B7-H5/VISTA	Cell Signaling	Cat#64953; RRID: AB_2799671
Anti-GAPDH	Santa Cruz Biotechnology	Cat#sc-32233; RRID: AB_627679
Goat anti-mouse 800	Li-Cor	Cat#926-32210; RRID: AB_621842
Goat anti-rabbit 680	Li-Cor	Cat#926-68071; RRID: AB_10956166
Anti-mouse Alexa Fluor 488	Thermo Scientific	Cat#A-11001; RRID: AB_2534069
Anti-rabbit Alexa Fluor 546	Thermo Scientific	Cat#A-11060; RRID: AB_2534107
Bacterial and virus strains
E. coli DH5alpha	Thermo Scientific	18265017
Biological samples
Tissue microarray containing ccRCC and metastasis specimens	Cruces Hospital	NA
Critical commercial assays
CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)	Promega	G3581
CellTiter-Glo® 3D Cell Viability Assay	Promega	G9683
Experimental models: Cell lines
HEK293	ATCC	CRL-1573
COS-7	ATCC	CRL-1651
786-O	ATCC	CRL-1932
Caki-1	ATCC	HTB-46
Oligonucleotides
Primer B7-H5 N49A Forward GTCCCGAGGGGCAGGCCGTCACCCTCACCTG	Life Technologies	A1561-2
Primer B7-H5 N49A Reverse CAGGTGAGGGTGACGGCCTGCCCCTCGGGAC	Life Technologies	A1561-2
Primer B7-H5 N91A Forward CCGGCCCATCCGCGCCCTCACGTTCCAGG	Life Technologies	A1561-2
Primer B7-H5 N91A Reverse CCTGGAACGTGAGGGCGCGGATGGGCCGG	Life Technologies	A1561-2
Primer B7-H5 N108A Forward GCCACCAGGCTGCCGCCACCAGCCACGACCT	Life Technologies	A1561-2
Primer B7-H5 N108A Reverse AGGTCGTGGCTGGTGGCGGCAGCCTGGTGGC	Life Technologies	A1561-2
Primer B7-H5 N128A Forward CCGACCACCATGGCGCCTTCTCCATCACCAT	Life Technologies	A1561-2
Primer B7-H5 N128A Reverse ATGGTGATGGAGAAGGCGCCATGGTGGTCGG	Life Technologies	A1561-2
Primer B7-H5 N135A Forward CATCACCATGCGCGCCCTGACCCTGCTGG	Life Technologies	A1561-2
Primer B7-H5 N135A Reverse CCAGCAGGGTCAGGGCGCGCATGGTGATG	Life Technologies	A1561-2
Primer B7-H5 N190A Forward CCAGGATAGTGAAGCCATCACGGCTGCAG	Life Technologies	A1561-2
Primer B7-H5 N190A Reverse CTGCAGCCGTGATGGCTTCACTATCCTGG	Life Technologies	A1561-2
Primer removing DYK/Flag Forward CCAAACTTTGAGGTCATCTGATAAACCCGCTGATCA	Life Technologies	A1561-2
Primer removing DYK/Flag Reverse TGATCAGCGGGTTTATCAGATGACCTCAAAGTTTGG	Life Technologies	A1561-2
Recombinant DNA
pcDNA3.1/C-DYK/Flag B7-H5	GenScript	NM_022153.1
Software and algorithms
GEPIA http://gepia.cancer-pku.cn	Peking University	RRID:SCR_018294
NetNGlyc http://www.cbs.dtu.dk/services/NetNGlyc/	CBS Prediction Servers	RRID:SCR_001570
Odessey Image Studio http://www.licor.com/bio/products/software/image_studio_lite/	Li-Cor	RRID:SCR_013715 v.4.0.21 software
ZEN Microscopy software https://www.zeiss.com/microscopy/en/products/software/zeiss-zen.html	ZEISS	RRID:SCR_013672
GraphPad Prism http://www.graphpad.com/	GraphPad	RRID:SCR_002798
SPSS Statistics http://www-01.ibm.com/software/uk/analytics/spss/	IBM	RRID:SCR_002865 v.29 software
Other
DMEM	Corning	Cat#10-017-CV
RPMI 1640 medium	Corning	Cat#10-040-CV
McCoy’s 5A medium	Corning	Cat#10-050-CV
FBS	Gibco	Cat#A5256701
L-Glutamine	Lonza	Cat#BE17-605E
Penicilin/Streptomycin	Lonza	Cat#DE17-602E
GenJet	SignaGen	Cat#SL100489
PBS	Corning	Cat#21-040-CV
M-PER lysis buffer	Thermo Scientific	Cat#78501
PhosStop	Roche	Cat#04 906 837 001
cOmplete protease inhibitor	Roche	Cat#04 693 132 001
PNGase F	New England Biolabs	Cat#P0704S
NuPAGE sample buffer	Thermo Scientific	Cat#NP0008
Prestained molecular marker	Sigma Aldrich	Cat#SDS7B2
Immobilon ®-FL PVDF Membrane	Merck	Cat# IPFL00010
Odyssey Blocking Buffer	Li-Cor	Cat# 927-70001
Mounting fluid with DAPI	Abcam	Cat# ab104139
PepMute	SignaGen	Cat#SL100566
siRNAs (siB7-H5#1–4)	Qiagen	Cat#1027416
dNTPs	Thermo Scientific	R0193
DpnI	Thermo Scientific	ER1701
Pwo	Roche	PWOPOL-RO
OWL HEP-1 transfer system	Thermo Scientific	Cat#HEP-1
Odyssey CLx Imager	Li-Cor	Cat#9140-09
ZEISS LSM880 AIRYSCAN	Zeiss	RRID:SCR_020925
AriaMx Real-Time PCR System	Agilent Technologies	Cat#G8830A
Mark™ Microplate Absorbance Reader	Bio-Rad	Cat#168-1130
Infinite® M plex	Tecan	Cat# 30190085
PTLink	Agilent Technologies	Cat#PT100
Dako Autostainer Link 48	Agilent Technologies	Cat#AS480