Fig. 1.

LPS induces NETosis comparable to PMA in dHL-60 cells. The percent of dHL-60 cells undergoing NETosis (n = 4) after stimulation with A) Different concentrations of LPS from P. aeruginosa and B) Different concentrations of PMA, compared to non-stimulated dHL-60 cells. Representative images (20×) showing nuclei (blue fluorescence) and DNA release labeling (green fluorescence) at 6 h after stimulation with C) LPS (100 μg/mL), D) PMA (100 nM) or E) non-stimulated cells are shown. NETs are quantified by total fluorescence object area (μm²/image) using Incucyte NETosis assay. Analysis was performed using two-way ANOVA with multiple comparisons to assess differences across concentrations versus non-stimulated cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. Data are graphed as box and whisker plots showing the max and min values.