Fig. 2.

Visualization of NETs MPO-DNA co-localization. Immunofluorescence staining was performed using antibodies targeting Myeloperoxidase (MPO), shown in green (FITC). DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole), shown in blue. This experiment was performed with dHL-60 cells stimulated with Lipopolysaccharide (LPS) from P. aeruginosa at A) 100 μg/mL and B) 10 μg/mL, C) PMA at 100 nM or D) non-stimulated. Cells were fixed after 2 h of stimulation. Representative images depict NETs structures with MPO-DNA co-localization. Images were captured at a magnification of 40×, and scale bar was set at 50 μm.