Fig. 2.

Alpha actinin-4 (ACTN4) regulates the proliferation of gastric cancer (GC) cells. (A) ACTN4 expression in three human GC cell lines and one human gastric mucosal epithelial cell line was detected by Western blotting; statistical plots are shown on the right. (B) Cell Counting Kit-8 (CCK-8) assay was used to detect the proliferation of two GC cell lines with stable knockdown of ACTN4 expression, and the measured absorbance values (absorption of optical density 450 (OD₄₅₀)) were used to draw line charts. (C) Cell cycle assay was used to detect the percentage of cells in G1/G0, S, and G2/M phases; statistical plots are shown on the right. (D) 5-Ethynyl-2′-deoxyuridine (EdU) staining was used to detect the DNA replication activity of the two GC cell lines after ACTN4 knockdown. Data are representative of three independent experiments. ^∗P < 0.05, ^∗∗P < 0.01 and ^∗∗∗P < 0.001. ns: no statistical significance. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; shNC: short hairpin negative control; DAPI: 4′,6-diamidino-2-phenylindole.