Fig. 5.

Polypyrimidine tract binding protein 1 (PTBP1) is co-expressed with alpha actinin-4 (ACTN4) and both are highly expressed and correlated in patients with gastric cancer (GC). (A) After the mice were sacrificed, the internal organs were removed, and the internal organs were fixed and sectioned for immunofluorescence (IF) experiments. ACTN4, PTBP1, and 4′,6-diamidino-2-phenylindole (DAPI) stainings were performed. (B) A brief schematic of creating the ptbp1 clustered regularly interspaced short palindromic repeat-associated protein 9 (Cas9)-knock out (KO) mouse model. (C) Representative images for multiplexed IF staining (ACTN4, green; PTBP1, pink; cytokeratin (CK), red; and DAPI, blue). (D, E) The number different labelled cells were counted separately to obtain their distribution in the tumour (D) and the proportional distribution of different cells corresponding to each sample (E). Each vertical bar represents a sample, and different colours represent different cells. (F) Differential expression analysis between cancer and adjacent tissues. The differences of PTBP1⁺CK⁺, ACTN4⁺CK⁺, and PTBP1⁺ACTN4⁺CK⁺ in GC tissues and their corresponding adjacent tissues were analysed and plotted (n = 75). (G) A cancer correlation matrix diagram; the features displayed on the X-axis and Y-axis are shown in the graph. Orange-red indicates a positive correlation, and blue indicates negative correlation. The darker the colour, the greater the correlation. In the association network diagram, the association was calculated based on Spearman's correlation analysis, and the co-expression relationship was mirrored by the selected threshold based on the selected significance identification drawing method and correlation coefficient. The red lines represent positive correlations. ^∗P < 0.05 and ^∗∗∗P < 0.001. WT: wild type; ATG: initiation codon; TAG: termination codon; sgRNA: single guide RNA.