Figure 2.

Primers used for the 5′-RACE approach. Sequence of the primers used for the 5′-RACE protocols. Specific primers derived from sequenced genes are not included. mRNA is isolated and cDNA synthesis is primed using oligo(dT) primer I, which contains a biotin residue at its 5′-end. CapFinder primer A or B is included in the first strand synthesis medium to tag the first strand cDNA with a known sequence at its 5′-end. To preamplify the capped cDNA a LA PCR reaction with 5′-Primer and 3′-Primer could be performed; both primers are devoid of the 3′-homopolymeric regions. In the 5′- and 3′-RACE protocols the latter primers are used together with a gene-specific reverse primer (GSRP) and gene-specific sense primers, respectively.