Figure 3.

5′-RACE experiments with different genes of the protozoan parasite E.histolytica. Using the 5′-RACE protocol outlined in Figure 1, a number of different 5′-regions of identified genes of the human protozoan parasite E.histolytica were detected. Gene-specific reverse primers (GSRP) were designed from the known sequences of the respective genes and used, together with the 5′-Primer and CapFinder cDNA to amplify the 5′-regions. In lanes 1 and 2 two different 5′-RACE products corresponding to the 5′-ends of cysteine protease cDNAs, in lanes 3 and 4 those of two thioredoxin cDNAs and in lanes 5 and 6 those of two protein disulphide isomerase cDNAs are separated. The smaller band in lane 5 represents a PCR artefact, as it appears even if only the GSRP is present in the PCR reaction.