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. 2000 Nov 15;28(22):e96. doi: 10.1093/nar/28.22.e96

Figure 4.

Figure 4

5′-RACE experiments with cysteine proteases of the protozoan parasite E.histolytica. 5′-Regions of the cysteine proteases CP 1 (lanes 1 and 3) and CP 112 (lanes 2 and 4) were amplified from cDNA derived from the protozoan parasites E.histolytica (lanes 1 and 2) and its close relative E.dispar (lanes 3 and 4). Gene-specific reverse primers (GSRP) were chosen that could be anticipated to amplify ∼900 (CP 1, lanes 1 and 3) or 1400 bp (CP 112, lanes 2 and 4). The 5′-RACE amplifications revealed single bands, whose identity was verified by sequencing.