Table 2.
Predicted Pathway Stoichiometry for Different C6 Oleochemicals Produced from Lactate
| Product; pathway | Overall equation | Product yield (mol P/mol lactate) | ATP yield (mol ATP/mol Lactate) | ΔGr°′ (kJ/mol) | ΔGr°′ per ATP (kJ/mol/mol ATP) | Yield (g P/g lactate) |
|---|---|---|---|---|---|---|
| Carboxylate; RBO | Lactate + 0.667 H+ + 0.333 ADP → CO2 + 0.667 H2 + 0.333 H2O + 0.333 hexanoate + 0.333 ATP | 0.333 | 0.333 | −26.75 | −80.24 | 0.431 |
| Alcohol; RBO + AdhE | Lactate + H+ + 0.333 ADP → CO2 + 0.667 H2O + 0.333 hexanol + 0.333 ATP | 0.333 | 0.333 | −45.18 | −135.53 | 0.382 |
| Alcohol; RBO + AOR | Lactate + H+ + 0.5 ADP → CO2 + 0.667 H2O + 0.333 hexanol + 0.5 ATP | 0.333 | 0.500 | −45.18 | −90.35 | 0.382 |
| Dicarboxylate; RBO + AlkBGT | Lactate + H+ + 0.667 NO2− + 0.333 ADP → 0.333 adipate + CO2 + 0.667 H2 + H2O + 0.333 N2 + 0.333 ATP | 0.333 | 0.333 | −228.21 | −684.62 | 0.539 |
| Diol; RBO + AlkBGT | Lactate + 0.5 NO2− + 1.25 H+ + 0.5 ADP → 0.25 1,6-hexanediol + 0.25 acetate + CO2 + H2O + 0.25 N2 + 0.5 ATP | 0.250 | 0.500 | −191.63 | −383.26 | 0.332 |
RBO = reverse beta-oxidation, AdhE = bifunctional alcohol dehydrogenase, AOR = aldehyde:ferredoxin oxidoreductase, AlkBGT = alkane 1-monooxygenase complex