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One-step growth analysis of SINV CP substitution mutants in BHK cells. BHK cells were infected with the indicated virus at a multiplicity of infection of 1. Media were replaced every 30 min for the first 2 h and then every hour for 12 h. Supernatant was collected at the indicated times, and released virus was assayed by titration on BHK cell monolayers at 37°C. The data represent averages of two independent experiments. The wild-type strain is Toto71.
