FIG. 1.

PLMVd rolling circle replication. The polymerization, hammerhead-mediated self-cleavage, and ligation steps are numbered 1 and 4, 2 and 5, and 3 and 6, respectively. The polarities of the strands are indicated in parentheses. The insets show schematic secondary structures for the PLMVd strands of both polarities according to a published previously model (9). For each strand, the nucleotide sequence and structure adopted at the ligation site are illustrated.