FIG. 2.
(A) Autoradiogram of 2D-PAGE analysis. RNA from PLMVd-infected (Siberian C cultivar) peach leaves mixed with in vitro-synthesized 32P-labeled C-PLMVd was fractionated on a 2D 5% polyacrylamide gel. The C- and L-PLMVd transcripts are indicated by arrows. The diagonal (dotted line), 5S RNA, and rRNA were revealed by silver staining. XC, xylene cyanol. (B and C) Typical 2D TLC autoradiograms corresponding to hydrolysis of the RNA species isolated from healthy and infected peach leaves, respectively. Nonradioactive mono- and dinucleotides were also cofractionated and are identified. The directions of migration are shown, and the origin (o) is indicated. These autoradiograms were overexposed in order to allow for the detection of any trace products.