TABLE 1.

Detection of the 2′,5′-linked dinucleotides

Peach cultivar or transcript	Infected plantsa	Extraction procedure	PLMVd bands isolated by 2D PAGEb	C/U detectedc
Cultivars
Siberian C	+	RNeasy	C	+
			L	−
		Tris-EDTA	C	+
	−	RNeasy	C	−
			L	−
Redhaven	+	PEG precipitationd	C	+
			L	−
Harrow Beauty	+	RNeasyd	C	+
			L	−
Agua	−	PEG precipitation	C	−
			L	−
Synthetic transcripts
3′,5′ linked			C	−
			L	−
			L + Ce	−
2′,5′ linked			C	+
			L	−

^a+, infected; −, not infected. 

^bL and C, L-PLMVd and C-PLMVd, respectively. 

^c+, detected; −, not detected. 

^dThis experiment was repeated in the presence of 5 mM EDTA in all extraction buffers, and the results were similar. 

^eA mixture of L- and C-PLMVd that was not gel purified but was treated directly.