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Gene knockout is recA-dependent. Plates demonstrating that wkB2 (PL60; top left) and wkB2 + recA::specR (PL269; top right) are successfully electroporated with a control kanR plasmid (pBTK519). The knockout construct amsE::kanR (gfPL147) is incorporated into wkB2 (bottom left), but not wkB2 + recA::specR (bottom right).
