Figure 5.

Several ACC cell lines and surgical samples do not express TERT. A, Univariate scatterplot of TERT transcriptional expression levels vs. ATRX transcriptional expression levels in the ACC cell line and surgical sample data set. B, Alternative lengthening of telomeres (ALT)-associated PML body formation. Representative confocal micrograph images of ALT cell line (U2OS) and ACC cell lines. Cells were fixed and immunofluorescently labeled with Abs against TERF2 and PML; TERF2 was stained green, PML red, and nuclei DAPI blue. C, DAXX and ATRX mutations in Colorado, Zurich, and Wurzburg data sets. Mutation scores in each dataset were collected and plotted; MUC-1 and CU-ACC2 have ATRX mutations, and JIL-2266 has DXAA mutation.