Figure 8.

The CU-ACC1 cell line is sensitive to temozolomide as it lacks MGMT and is MMR proficient, whereas CU-ACC2 cells are resistant to temozolomide because of MMR deficiency. A, CU-ACC1 and CU-ACC2 do not express MGMT transcripts. Univariate scatterplot of MGMT transcriptional expression levels vs. MGMT gene promoter methylation levels in the ACC cell line dataset. B, MGMT protein expression levels in CU-ACC1, CU-ACC2, NCI-H295R, and SW-13. Proteins were extracted from each cell line, and MGMT expression was assessed by Western blotting. C, The CU-ACC2 cell line is defective in MMR due to lack of expression of the MSH2 gene. Univariate scatter plot of MHS2 transcript levels vs. MSH2 gene copy number in the ACC cell lines data set. D, Dose–response curves of temozolomide in CU-ACC1, CU-ACC2, NCI-H295R, and SW-13. Cell viability was assessed after 72 hours under the indicated drug concentrations by CellTiter-Glo assay.