Fig. 1.

SQ-diEG attenuated cellular proliferation in B16F10 cells and SK-MEL-28 cells. A The compound structure of 2-[2-(2-hydroxyethoxy)ethoxy]-3-hydroxysqualene (SQ-diEG). B The effect of SQ-diEG on B16F10 cell viability was measured by MTT assay. C Immunostaining of Ki67 in B16F10 cells. D The effect of SQ-diEG on cell cycle arrest in B16F10 cells after 24 h treatment. E The effect of SQ-diEG on cell cycle arrest in B16F10 cells after 48 h treatment. F The effect of SQ-diEG on cell cycle arrest in B16F10 cells after 72 h treatment. Cell distribution in cell cycle was expressed as % total (pink indicates G0/G1 phase, green indicates S phase, blue indicates G2/M phase). G The effect of SQ-diEG on SK-MEL-28 cell viability was measured by MTT assay. H MitoTracker Red CM-H2XRos staining of mitochondria in SK-MEL-28 cells. All the data were analyzed and expressed as mean ± standard deviations (SD) (n = 3). The level of significance between two value sets (ctrl cells vs. treated cells) was determined by student's t test: * P < 0.05, **P < 0.01, ***P < 0.0001