FIG. 3.

Effects on termination of changes in the U-tract length and position. (A) Sequence of the WT M/SH gene junction aligned with sequences of mutant replicons having alterations in the U-tract length and position. Replicon names (left) give the sequences (3′ to 5′ in the negative sense) of the central region and the U tract, separated by a dash. The sequences are represented as in Fig. 2B. (B) Analysis of viral RNA synthesis directed by WT and the indicated mutant replicons. The nomenclature for RNA species (right) and the experimental procedures are the same as for Fig. 2C. (C) TE at GE1, calculated for each replicon by densitometric analysis of fluorograms as described in Materials and Methods. The average percent TE values (bars) and standard deviations (error bars) were determined for all replicons from at least three independent experiments.