Figure 3. Transduction of Ad-H11-KI failed to induce apoptosis.

Myocytes were transduced with Ad-LacZ, Ad-H11 or Ad-H11-KI (MOI=10–100). (A) Cell lysates were subjected to immunoblot analyses with anti-H11 antibody (upper panel) and immunocomplex protein kinase assays using myelin basic protein (MBP) as a substrate (lower panel). (B) Phase-contrast microscope images of myocytes at 48 h. (C) At 48 h, apoptosis was quantified by Cell Death ELISAs. A₄₀₅ (OD405) in untransduced myocytes was designated as 1.0. Each column represents the mean for three experiments performed in duplicate. NT, no transduction. *P<0.001 versus NT. n.s., not significant.