Figure 4. Cardiac hypertrophy by high doses of H11 is unmasked by caspase 3 inhibitor treatment.

Myocytes were transduced with Ad-LacZ or Ad-H11 at MOI=100 (A) or at the indicated MOI (B, C); 12 h after transduction, myocytes were incubated with or without DEVD-CHO (150 μM) for 48 h. (A) Phase-contrast microscope images. NT, no transduction. (B) Results of Cell Death ELISAs are shown. The A₄₀₅ (OD405) in untransduced myocytes was designated as 1.0. Results are representative of three experiments performed in duplicate. (C) Total protein content/DNA content was determined from three experiments performed in triplicate each. The data from myocytes without adenovirus transduction or DEVD-CHO (white bar) were designated as 1.0. NT, no transduction. *P<0.001 versus NT.