Table 1. EPR parameters of the Fe/S EPR signals from bovine and human milk XOR.
The g-values, line widths (Δi, mT) and occupancies are those used in the simulations of Figure 6. Gaussian line shapes were assumed for Fe/S I and III, and Lorenzian for Fe/S II. Occupancies of the centres correspond to molar ratios of the different signal-giving species relative to that of Fe/S II, which is taken as 1.00 for each enzyme. Errors in g-values were estimated from repetitive measurements.
| Enzyme | Signal | g1 | Δ1 | g2 | Δ2 | g3 | Δ3 | Occupancy |
|---|---|---|---|---|---|---|---|---|
| Bovine | Fe/S I | 2.0230±001 | (1.3) | 1.933±0.001 | (1.0) | 1.899±0.001 | (1.2) | 0.85 |
| Fe/S II | 2.122±0.005 | (4.5) | 2.005±0.003 | (2.5) | 1.888±0.005 | (3.8) | 1.00 | |
| Fe/S III | 2.060±0.001 | (1.2) | 1.930* | (1.2) | 1.857±0.001 | (1.2) | 0.08 | |
| Human | Fe/S I | 2.021±0.001 | (1.1) | 1.930±0.001 | (1.1) | 1.903±0.001 | (1.3) | 0.51 |
| Fe/S II | 2.105±0.005 | (4.7) | 2.000±0.005 | (2.5) | 1.89* | (4.2) | 1.00 | |
| Fe/S III | 2.059±0.001 | (1.2) | 1.93* | (1.2) | 1.854±0.001 | (1.2) | 0.20 |
* Errors not calculated.