Table 1.
Strains and plasmids
| Strains and plasmids | Description | Reference |
|---|---|---|
| E. coli | ||
| DH5α λpir | Host for plasmid amplification: supE44, ΔlacU169 (φ80 lacZΔM15), hsdR17 (rk−mk+), recA1, endA1, thi1, gyrA, relA, λpir lysogen | Biomedal Life Sci., Seville, Spain |
| CC118λpir | Mating donor strain: Δ(ara-leu), araD, ΔlacX174, galE, galK, phoA, thi1, rpsE, rpoB, argE (Am), recA1, lysogenic λpir | [94] |
| HB101 | Mating helper strain: SmR, hsdR-M+, pro, leu, thi, recA | [95] |
| Plasmids | ||
| KT2440 | Wild type | [2] |
| ΔscpC | KT2440 derivative: ΔscpC (PP_0154) | This work |
| ΔPP_5266 | KT2440 derivative: ΔPP_5266 | This work |
| ΔacsAI ΔacsAII | KT2440 derivative: ΔacsAI (PP_4487) ΔacsAII (ΔPP_4702) | This work |
| ΔaldBI ΔaldBII | KT2440 derivative: ΔaldBI (ΔPP_0545) ΔaldBII (or pedI, ΔPP_2680) | This work |
| Plasmids | ||
| pGNW2 | Suicide plasmid for integration/deletion: KmR, oriR6K, lacZα with two flanking I-SceI sites, P 14 g→msfGFP | [74] |
| pSEVA6213S | Helper plasmid: GmR, oriV (RK2), xylS, PEM7→I-SceI | [74] |