Table 1. GlcN and gangliosides GM2 and GM1 block the cell-cycle progression of mesangial cells.
RMC were treated with GlcN (0.5 or 5 mM) or cultured in the presence of GM2 or GM1 (60 μM) for 48 h. Cells were then collected and analysed by flow cytometry as described in the Experimental section. Results are expressed as percentage of cells in each phase of the cell cycle: G0/G1, S and G2/M. Values are the means±S.E.M. for five independent experiments.
G0/G1 phase | S phase | G2/M phase | |
---|---|---|---|
Control | 58±2 | 22±1 | 19±1 |
GlcN | |||
0.5 mM | 71±1 | 13±1 | 16±1 |
5 mM | 54±1 | 15±1 | 29±2 |
GM2 | 79±2 | 9±1 | 12±1 |
GM1 | 79±1 | 8±1 | 12±1 |