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A given expression plasmid containing each mCAR mutant (0.2 μg) was co-transfected into HepG2 cells with (NR1)5-tk-luciferase plasmid (0.1 μg) and pRL-SV40 (0.01 μg) for 16 h. Then cells were treated with DMSO or TCPOBOP (250 nM) for an additional 24 h and subjected to luciferase assay. The results are expressed as the means±S.D. from three independent experiments.
