Figure 9. Interaction of hPXR and mVDR with SRC-1.

Mammalian two-hybrid assay was performed as described in the Experimental section. The VP16-fused wild-type (Wt) and mutated receptors (0.2 μg) were co-transfected with Gal–SRC-1 (0.2 μg) and pG5luc vector (0.2 μg) into HepG2 cells for 16 h. Cells were treated with DMSO or rifampicin (Rif; 10 μM) for hPXR (A) and EtOH or vitamin D₃ (VD3; 100 nM) for mVDR (B) for further 24 h. Luciferase activities (means±S.D.) were determined as described for previous experiments.