Figure 9. Co-immunoprecipitation of spectrin peptide, Tes and EVL.

Lysates of COS-7 cells overexpressing FLAG-tagged spectrin α8-α11 peptide, V5-tagged Tes and EVL were immunoprecipitated with anti-FLAG M2 beads. The complexes eluted by SDS were analysed by Western blotting using antibodies directed against EVL (left panel); after stripping, the blot was probed with antibodies directed against the V5-epitope to reveal Tes (right panel). When expressed separately, EVL and Tes each immunoprecipitates with the spectrin peptide. When EVL and Tes are co-expressed, only EVL is immunoprecipitated by the spectrin peptide.