Scheme 1. Metabolite library coverage.

We generated a MCF derivatization metabolite library, covering much of central carbon metabolism and nearly all of amino acid biosynthesis pathways. We show the metabolic network of amino acid biosynthesis in S. cerevisiae during aerobic growth, highlighting (in grey) metabolites in our library. Continuous arrows indicate a one-step reaction, and broken arrows indicate a series of biochemical reactions where the numbers indicate the reaction steps in the pathway. This illustrative figure does not include all metabolites present in the library.