Figure 4. Glycosidase digestion of recombinant MT-SP1/matriptase expressed in COS-1 cells.

Concentrated conditioned medium of COS-1 cells expressing MT-SP1wt was incubated with (+) or without (−) glycopeptidase F (GPF), endo H or O-glycosidase (O-gly) and neuraminidase (Neu). The digested samples were separated by SDS/PAGE (12% polyacrylamide), and Western-blot analysis was performed with Spr992. The molecular-mass standards are indicated on the left in kDa.