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. 2005 May 24;388(Pt 2):697–703. doi: 10.1042/BJ20050160

Figure 4. Effect of RGT1 gene disruption on the expression of SUC2.

Figure 4

(A) The wild-type yeast strain (DBY1315) and its isogenic rgt1 null mutant were grown using 2% glucose (open bars) or 3% ethanol plus 0.05% glucose (solid bars) as carbon sources, until the A600 reached 1.0 [3.0 mg (wet weight)/ml]. Invertase activity was assayed in whole cells. The average values of the results obtained for four independent experiments have standard errors ≤10%. (B) Yeast rgt1 mutant strain expressing Hxk2–GFP (a and b) from the plasmid YEp352/HXK2::gfp and Mig1–GFP (c and d) from the plasmid YEp352/MIG1::gfp were grown on SD-Ura− medium supplemented with glucose or ethanol as carbon source and fluorescence was imaged. The cells were stained with DAPI for DNA and then imaged for GFP fluorescence and for DAPI fluorescence by phase-contrast optics.