Fig. 3. Negative impacts of parental age on spat oyster survival to OsHV-1 challenge.

(A) In the laboratory, we optimized conditions for viral replication (seawater at 21°C, food ad libitum) and found lower survival chance in offspring (n = 4325) from both old parents. (B) Offspring exposed to natural conditions (n = 3407) in Bay of Brest had similar mortality risks correlated with laboratory measures (dot colors, means ± SE of familial average; solid line, model predictions surrounded by 95% CIs).