TABLE 1.
CTLp frequency of MmL-3 under different culture conditions
| Culture conditions | No. of CTLp/106 PBMC (95% confidence limits)
|
||
|---|---|---|---|
| Mock | Vac-SC | Vac-SIVgag | |
| Different stimulators added | |||
| 10% autologous PBMC (unfixed) | 1 (3–16) | 9 (5–18) | 211 (163–273) |
| 10% BLCL/psoralen/UV fixed | 3 (1–9) | 5 (2–12) | 29 (19–44) |
| 10% BLCL/paraformaldehyde fixed | 4 (1–11) | 3 (1–9) | 75 (56–101) |
| Time of LDA culture | |||
| 9 days | 1 (0–8) | 4 (1–11) | 11 (6–21) |
| 14 days | 17 (10–29) | 2 (0–8) | 187 (144–241) |
| 16 days | 6 (2–14) | 1 (0–8) | 357 (271–470) |
| 21 days | 13 (7–24) | 10 (5–20) | 195 (151–253) |
| IL-2 addeda | |||
| 1× (normal regimen) | 13 (7–23) | 5 (2–12) | 116 (88–151) |
| 0.5× | 6 (3–14) | 7 (3–15) | 97 (74–128) |
| 2× | 129 (99–168) | 111 (85–145) | 433 (327–574) |
| 4× | 201 (155–260) | 165 (128–214) | 334 (255–439) |
| Irradiated feeder cells added | |||
| Human PBMC | 1 (0–8) | 14 (4–17) | 139 (76–132) |
| Rhesus PBMC | 7 (3–16) | 16 (10–27) | 61 (45–84) |
| Mouse splenocytes | 5 (2–12) | 58 (42–79) | 18 (11–29) |
| None | 2 (0–8) | 1 (0–8) | 147 (114–191) |
| IL-7 added to LDA cultureb | |||
| No | 15 (9–26) | 9 (5–19) | 157 (121–260) |
| Yes | 9 (5–19) | 5 (2–12) | 187 (145–242) |
| % of autologous PBMC infectedc | |||
| 10% | 7 (3–15) | 8 (4–16) | 197 (153–253) |
| 20% | 13 (9–24) | 7 (3–15) | 228 (177–293) |
| 100% | 22 (14–35) | 12 (7–22) | 92 (70–121) |
a
IL-2 feeding schedule and normal concentration are detailed in Materials and Methods.
b
IL-7 was added on day 0 at 330 U/ml.
c
The percentages indicate the portions of PBMC in the cultures that were infected with Vac-SIVgag for restimulation.