TABLE 2.
Kinetics of p11C tetramer-positive CTL expansion in cultures
| Monkey no. | Status | Restimulation methoda | % p11C tetramer stainingb on day of culture:
|
|||
|---|---|---|---|---|---|---|
| 0 | 8 | 10 | 14 | |||
| 94–98 | SIV+ | Vac-SIVgag | 1.25 | 9.90 | 17.73 | 31.66 |
| p11C peptide | 1.25 | 11.12 | 11.70 | 12.76 | ||
| 061F | Naïve | Vac-SIVgag | 0.05 | 0.48 | 0.24 | 0.91 |
| p11C peptide | 0.05 | 0.19 | 0.07 | 0.17 | ||
a
The cultures were restimulated with 20% autologous PBMC infected with Vac-SIVgag or with p11C peptide at 1 μg/ml.
b
The lymphocytes were gated on CD3+ and CD8+ populations.