FIG. 1.

Detection of interactions between CaMV P3 and either purified virus particles (A) or preCP (B). E. coli extracts containing P3 (+) or not (−) were fractionated by SDS-PAGE (18% polyacrylamide) and transferred onto a nitrocellulose membrane. P3 was detected with anti-P3 antibodies (lanes 1). (A) Strips from the same blot were incubated either with purified virions (lanes 2) or with buffer (lanes 3), then washed, and treated with anti-CP antibodies raised against the 37-kDa CP form. (B) Strips were incubated with bacterial extracts containing preCP (lanes 2) or not (lanes 3), then washed, and treated with anti-CP antibodies.