FIG. 3.

Analysis of genomic RNA incorporation into WT and NC mutant SIV(Mne) virions. Virus was pelleted from clarified supernatants of transfected 293T cells, and viral RNA was isolated by phenol-chloroform extraction–ethanol precipitation. Samples, equalized for pelletable RT activity, were probed with the pRB85 vector containing full-length SIV(Mne) that had been linearized with XhoI and then labeled with ³²P (23). The mock control lane contains an RNA preparation from carrier DNA-transfected 293T supernatants. Full-length genomic RNA (9.6 kbp) indicated on the left was quantitated by phosphorimaging (PI) and quantitative real-time RT-PCR (PCR). Positions of RNA markers are shown on the right. ND, not determined.