Extended Data Fig. 9. Stroke initiates the intrinsic coagulation cascade at atherosclerotic plaques.

All analyses were performed on CCA lysates in mice with stenotic CCA plaques after TS surgery in HFD fed ApoE^−/−. a. Representative immunoblot of activated Factor XII (F. XIIa) 1 w after stroke or sham surgery. b. Representative immunoblot micrograph of F. XIIa in CCA lysates 24 h after i.v. DNA challenge. c. Representative immunoblot of the F. XIIa in CCA lysates 24 h after stroke in mice treated with control treatment or caspase-1 inhibition (VX765). Corresponding quantification of F. XIIa intensity normalized to β-actin in CCA lysates 1 w after stroke in control- or caspase-1 inhibitor- treated mice (U test, n = 7 per group). d. Representative gelatin zymography of CCA lysates for MMP activity in mice 24 h after sham, stroke or stroke + rhDNase I treatment (left). Representative immunoblot of F. XIIa in +TS CCA lysates 24 h after surgery (right). Raw membrane images of immunoblots with cropping indication can be found in Supplementary Fig. 1j–n.