FIG. 2.

Effect of various RNA templates on endonuclease activity analyzed by 15% PAGE in 7 M urea. Lane 1, minus enzyme; lane 2, no added RNA template; lane 3, 3′ vRNA sequence (5′ ACC CUG CUU UUG CU 3′); lane 4, 5′ vRNA (5′ AGU AGA AAC AAG GGU 3′); lane 5, vRNA (Fig. 1A); lane 6, cRNA (Fig. 1B); lane 7, vRNA lacking U track (Fig. 1C); lane 8, cRNA-like template with U track (Fig. 1D); lane 9, THOV vRNA (see Materials and Methods); lane 10, chemically synthesized 5′ ³²P-end-labeled 14-nt RNA size marker (labeled M) (12) (see lane 3). Minor bands in lanes 1 to 9 are probably nonspecific RNase degradation products. Minor bands in lane 10 represent contaminating RNA. S, 67-nt-long substrate; P, 12-nt-long cleavage product.