FIG. 3.

DHBV L-protein expression is sufficient for receptor down-regulation. (A) Indirect immunofluorescence of PDHs infected for 4 h with Ad-L (upper panels) or DHBV (lower panels). At day 6 postinfection, the cells were fixed and stained with an anti-L antibody and an antiserum against gp180/CPD (secondary antibodies were rhodamine and fluorescein conjugated for the right and left panels, respectively). Double fluorescence was analyzed by confocal microscopy (63× lens; sequential scans of the two fluorescent channels). (B) Anti-gp180/CPD Western blot of lysates of PDHs, infected on day 4 postplating with Ad-GFP or Ad-L. w/o, control cells without adenovirus infection. The position of gp180/CPD is indicated (arrow).