Table. 2.

Activity of PPARδ/sEH fine-tuning molecules on the targets of interest

Fine-tuning molecule	EC50(PPARδ) [µM]	IC50(sEH) [µM]	Tanimoto
	0.016	>30	0.17; 0.13; 0.13
	0.022	>30	0.20; 0.15; 0.16
	0.009	>30	0.13; 0.11; 0.11
	>50	0.005	0.55; 0.25; 0.27
	>50	0.097	0.38; 0.19; 0.19
	>50	<0.001	0.11; 0.13; 0.13

Commercially available fine-tuning molecules for PPARδ/sEH were experimentally confirmed selective for their annotated bioactivity over the respective second target in the assays used to characterize the dual PPARδ/sEH ligand designs 7–9. Data are the mean; n = 3. Tanimoto refers to the fine-tuning molecules’ similarity to the experimentally tested dual ligand designs 7–9.